... is a molecular cell biologist with interests in the cytoskeleton and membrane trafficking. He graduated from Cambridge University (2002) with a degree in biochemistry, and completed doctoral studies under the supervision of John Kendrick-Jones at the Laboratory of Molecular Biology, Cambridge (2006). He carried out postdoctoral studies at Yale University (2007) and the Max F. Perutz Laboratories in Vienna (2008-2014) in the laboratory of Graham Warren. Brooke joined the Biocenter in April 2015. He is the writer of the Total Internal Reflection science blog.
since 2015 Junior group leader, Cell and Developmental Biology, University of Würzburg
2008-2015 Postdoc, Max F. Perutz Laboratories, Vienna, Austria
2007 Postdoc, Yale University, USA
2006 PhD, Laboratory of Molecular Biology, Cambridge, UK
Tel ++49 93131 83556
The cytoskeleton of Trypanosoma brucei is essential for its viability, and contributes to a number of cellular processes including membrane trafficking and motility. Several discrete cytoskeletal structures are clustered around the neck of the flagellar pocket, an invaginated subdomain of the plasma membrane which is the sole site of endo- and exocytosis in T. brucei. One of these structures is a multiprotein complex defined by the repeat-motif protein TbMORN1. The morphology, composition, and function of this complex is the primary focus of Brooke's research. Experimental approaches involve a combination of light microscopy, fluorescence microscopy and electron microscopy, together with biochemical and functional assays. TbMORN1 was the focus of a highly-successful screen using proximity-dependent biotinylation (BioID), and this technique remains a cornerstone of the lab's work.
The second main theme in the laboratory is focused around the actomyosin system in trypanosomes, and specifically their myosin motor proteins. T. brucei exhibits a dramatically reduced actomyosin complement relative to metazoan cells, making it an ideal model system for some fundamental questions in eukaryotic cell biology.
Morriswood B, Engstler M. Let's get fISSical: fast in silico synchronization as a new tool for cell division cycle analysis. (2017) Parasitology. Feb 7:1-14.
Cicova Z, Dejung M, Skalicky T, Eisenhuth N, Hanselmann S, Morriswood B, Figueiredo LM, Butter F, Janzen CJ. (2016) Two flagellar BAR domain proteins in Trypanosoma brucei with stage-specific regulation. Sci Rep. 6:35826.
Vidilaseris K, Lesigang J, Morriswood B, Dong G. Assembly mechanism of Trypanosoma brucei BILBO1 at the flagellar pocket collar. (2015) Commun Integr Biol.8(1):e992739.
Morriswood B. Form, Fabric, and Function of a Flagellum-Associated Cytoskeletal Structure. (2015) Cells. 4(4):726-47.
Morriswood B, Schmidt K. A MORN Repeat Protein Facilitates Protein Entry into the Flagellar Pocket of Trypanosoma brucei. (2015) Eukaryot Cell. 14(11):1081-93.
Vidilaseris K, Shimanovskaya E, Esson HJ, Morriswood B, Dong G. Assembly mechanism of Trypanosoma brucei BILBO1, a multidomain cytoskeletal protein. (2014) J Biol Chem. 289(34):23870-81.
Vidilaseris K, Morriswood B, Kontaxis G, Dong G. Structure of the TbBILBO1 protein N-terminal domain from Trypanosoma brucei reveals an essential requirement for a conserved surface patch. (2014) J Biol Chem. 289(6):3724-35.
Morriswood B, Warren G. Cell biology. Stalemate in the Golgi battle. (2013) Science. 341(6153):1465-6.
Morriswood B, Havlicek K, Demmel L, Yavuz S, Sealey-Cardona M, Vidilaseris K, Anrather D, Kostan J, Djinovic-Carugo K, Roux KJ, Warren G. Novel bilobe components in Trypanosoma brucei identified using proximity-dependent biotinylation. (2013) Eukaryot Cell. 12(2):356-67.
Esson HJ, Morriswood B, Yavuz S, Vidilaseris K, Dong G, Warren G. Morphology of the trypanosome bilobe, a novel cytoskeletal structure. (2012) Eukaryot Cell. 11(6):761-72.