Deutsch
    Chair of Cell and Developmental Biology (Zoology I)

    BENAVENTE LAB

    We investigate the molecular organization, function and evolutionary history of the mammalian meiotic chromosome

    Research synopsis

    Meiosis is a special type of cell division that is restricted to the germ line. It consists of two successive cellular divisions that take place without intervening DNA replication. Meiosis is the basis for sexual reproduction in that it enables the generation of haploid cells. Furthermore, it represents the largest source of genetic variability. Errors in meiosis are a major cause of aneuploidy and fertility problems also in humans.

    During the extended meiotic prophase of the first meiotic division major changes in nuclear architecture take place that are critical for the meiotic process. At early prophase (leptotene stage), homologous chromosomes attach their telomeres to the nuclear envelope. Subsequently, homologous chromosomes, which initially occupy different nuclear territories, move and initiate stable pairing between them (zygotene stage) via a structure called synaptonemal complex (SC). When completely assembled at pachytene stage, each SC extends all along a chromosome bivalent. During diplotene and diakinesis, the SC disassembles and the homologous chromosomes desynapse.

    SCs are evolutionarily conserved meiosis-specific karyoskeletal structures which play a key role during synapsis of homologous chromosomes, chiasmata formation and chromosome segregation. SCs show a tripartite ladder-like organization characterized by the presence of two lateral elements (LEs) to which chromatin loops of the individual homologous chromosomes are attached. The third component - the central region (CR) - holds the SC together and is located in the gap between LEs. The CR is composed of a central element (CE) and numerous perpendicularly-oriented transverse filaments (TFs) that link the LEs with the CE. The major protein components of mammalian SCs have been already characterized: the meiosis-specific proteins SYCP1, 2 and 3. More recently, CE-specific proteins have been identified (i.e. SYCE1, 2, 3, 4 and 5).

    In order to investigate the molecular architecture and dynamics of the synaptonemal complex, we are applying super-resolution imaging and electron microscopic tomography to meiotic chromosomes and their telomeres of wildtype and selected knockout mice.

    Recent publications

    Spindler, M.C., F. Helmprobst, C. Stigloher & R. Benavente: EM tomography of LINC complexes. Methods Mol. Biol. (2017, in press).

    da Cruz, I., R. Rodríguez-Casuriaga, F. Santiñaque, J. Farías, G. Curti, A. Capoano, G. Folle, R. Benavente, J.R. Sotelo-Silveira & A. Geisinger: Transcriptome analysis of highly purified mouse spermatogenic cell populations: Gene expression signatures switch from meiotic-to postmeiotic-related processes at pachytene stage. BMC Genomics 17: 294 (2016).

    Schücker, K., T. Holm, C. Franke, M. Sauer & R. Benavente: Elucidation of synaptonemal complex structure by super-resolution imaging with isotropic resolution. Proc. Natl. Acad. Sci. USA 112: 2029-2033 (2015).

    Link, J., D. Jahn, J. Schmitt, E. Göb, J. Baar, S. Ortega, R. Benavente* & M. Alsheimer*: The meiotic nuclear lamina regulates chromosome dynamics and promotes efficient homologous recombination in the mouse. PLoS Genet. 9: e1003261 (2013).

    Fraune, J., C. Brochier-Armanet, M. Alsheimer & R. Benavente: Phylogenies of central element proteins reveal the dynamic evolutionary history of the mammalian synaptonemal complex: Ancient and recent components. Genetics 195: 781-793 (2013).

    Fraune, J., M. Alsheimer, J.N. Volff, K. Busch, S. Fraune, T.C. Bosch & R. Benavente: Hydra meiosis reveals unexpected conservation of structural synaptonemal complex proteins across metazoans. Proc. Natl. Acad. Sci. USA 109: 16588-16593 (2012).

    Schramm, S., J. Fraune, R. Naumann, A. Hernandez-Hernandez, C. Höög, H.J. Cooke, M. Alsheimer & R. Benavente: A novel mouse synaptonemal complex protein is essential for loading of central element proteins, recombination and fertility. PLoS Genet. 7: e1002088 (2011).

    RESEARCH TOPICS

    Meiotic Chromosomes

    Chromosome dynamics during meiosis is evolutionarily conserved in metazoans

    Meiotic Gene Expression

    A collaborative project with colleagues in Uruguay to elucidate stage-specific gene expression of meiotic cells

    Contact

    Lehrstuhl für Zoologie I - Zell- und Entwicklungsbiologie
    Am Hubland
    97074 Würzburg

    Phone: +49 931 31-84250
    Fax: +49 931 31-84252
    Email

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    Hubland Süd, Geb. B1 Hubland Nord, Geb. 32 Julius-von-Sachs-Platz 2 Fabrikschleichach Hubland Süd, Geb. B2 Campus Medizin