Dr. Susanne Kramer
Phone: 0931 31 86785
Susanne Kramer is a molecular biologist with a particular interest in mRNA metabolism and posttranscriptional regulation of gene expression. Susanne has received her degree in biochemistry at the Free University in Berlin in 2000, where she also started her PhD project in the laboratory of Professor Michael Boshart. She received her doctoral degree in 2005 at the LMU in Munich, followed by six years of postdoctoral studies at the Department of Biochemistry in Cambridge (UK) in the lab of Professor Mark Carrington. Susanne has joined the Biocenter in November 2011.
Susanne has used the African Trypanosome as a model system for 16 years and worked on two different aspects:During her PhD time she has characterized the trypanosome homologue to a protein kinase A, an enzyme that was suspected to mediate the cAMP signal that has been shown to mediate differentiation between the two different bloodstream form life-cycle stages of Trypanosoma brucei. Surprisingly, the kinase turned out not to be activated by cAMP in vitro. This unexpected result lead Susanne to develop an in vivo kinase assay by transgenically expressing a mammalian PKA reporter substrate. The assay confirmed the insensitivity to cAMP and was used to screen for alternative activators. During her Postdoc time in Cambridge, Susanne became interested in understanding post-transcriptional control mechanisms: trypanosomes have almost no transcriptional control and are perfect model organisms for this research area. In particular, Susanne became very interested in RNP-granules, small ribonucleoprotein particles that can be best described as an ‘out-of-translation’ place for mRNAs with a potential function in mRNA storage, sorting and decay. The exact function of RNP granules still remains somewhat mysterious. Susanne believes that understanding RNP granules, in particular the mechanisms that determine whether an individual mRNA is transferred into or out of the granules, are essential to understand translational control, which may be the predominant mechanism of gene expression control in eukaryotes. At the Biocenter in Würzburg, Susanne has developed methods for RNA granule purification. With the granule’s protein and RNA content in hand Susanne is currently analysing granule function using a combination of biochemical, cell biological and molecular biological methods. … MORE
Five important publications
Kramer S. 2016. Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution. Nucleic Acids Res, in press
Fritz M, Vanselow J, Sauer N, Lamer S, Goos C, Siegel TN, Subota I, Schlosser A, Carrington M and Kramer S. 2015. Novel insights into RNP granules by employing the trypanosome’s microtubule skeleton as a molecular sieve. Nucleic acid research 43:8013-32.
Krüger T, Hofweber M, Kramer S. 2013. SCD6 induces ribonucleoprotein granule formation in trypanosomes in a translation-independent manner, regulated by its Lsm and RGG domains. Mol Biol Cell 24: 2098-2111.
Kramer S, Marnef A, Standart N, Carrington M. 2012. Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate. J Cell Sci 125: 2896-2909.
Kramer S, Queiroz R, Ellis L, Hoheisel JD, Clayton C, Carrington M. 2010. The RNA helicase DHH1 is central to the correct expression of many developmentally regulated mRNAs in trypanosomes. J Cell Sci 123: 699-711.